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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-85479 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOX4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution.
- Concentration
- 1.84 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NOX4 in A) Jurkat cells using NOX4 polyclonal antibody (Product # PA5-85479) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NOX4 in various whole cell extracts using NOX4 polyclonal antibody (Product # PA5-85479) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NOX4 was performed by separating 30 µg of non-transfected (–) and transfected (+) unboiled 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NOX4 Polyclonal Antibody (Product # PA5-85479) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NOX4 was performed by separating 30 µg of SH-SY-5Y whole cell and nuclear extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NOX4 Polyclonal Antibody (Product # PA5-85479) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of NOX4 in A431 cells using NOX4 polyclonal antibody (Product # PA5-85479) at a dilution of 1:500. Sample was then incubated with Hoechst secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of NOX4 in A431 cells using NOX4 polyclonal antibody (Product # PA5-85479) at a dilution of 1:500. Sample was then incubated with Hoechst secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NOX4 in paraffin-embedded rat kidney using NOX4 polyclonal antibody (Product # PA5-85479) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NOX4 in paraffin-embedded human colon carcinoma tissue using NOX4 polyclonal antibody (Product # PA5-85479) at a dilution of 1:250.
