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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF148 - Provider product page
- Provider
- R&D Systems
- Product name
- Human BCAM Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human BCAM in direct ELISAs and Western blots. In these formats, approximately 2% cross-reactivity with recombinant human (rh) ALCAM is observed and less than 1% cross-reactivity with rhPECAM, rhEpCAM, rhICAM-1, rhICAM-2, rhICAM-3, and rhVCAM-1 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
CAA58449- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Glycophorin-C sialylation regulates Lu/BCAM adhesive capacity during erythrocyte aging.
Hypoxia-enhanced adhesion of red blood cells in microscale flow.
Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment.
Identification and characterization of Lutheran blood group glycoprotein as a new substrate of membrane-type 1 matrix metalloproteinase 1 (MT1-MMP): a systemic whole cell analysis of MT1-MMP-associating proteins in A431 cells.
Klei TRL, de Back DZ, Asif PJ, Verkuijlen PJJH, Veldthuis M, Ligthart PC, Berghuis J, Clifford E, Beuger BM, van den Berg TK, van Zwieten R, El Nemer W, van Bruggen R
Blood advances 2018 Jan 9;2(1):14-24
Blood advances 2018 Jan 9;2(1):14-24
Hypoxia-enhanced adhesion of red blood cells in microscale flow.
Kim M, Alapan Y, Adhikari A, Little JA, Gurkan UA
Microcirculation (New York, N.Y. : 1994) 2017 Jul;24(5)
Microcirculation (New York, N.Y. : 1994) 2017 Jul;24(5)
Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment.
Rodin S, Antonsson L, Niaudet C, Simonson OE, Salmela E, Hansson EM, Domogatskaya A, Xiao Z, Damdimopoulou P, Sheikhi M, Inzunza J, Nilsson AS, Baker D, Kuiper R, Sun Y, Blennow E, Nordenskjöld M, Grinnemo KH, Kere J, Betsholtz C, Hovatta O, Tryggvason K
Nature communications 2014;5:3195
Nature communications 2014;5:3195
Identification and characterization of Lutheran blood group glycoprotein as a new substrate of membrane-type 1 matrix metalloproteinase 1 (MT1-MMP): a systemic whole cell analysis of MT1-MMP-associating proteins in A431 cells.
Niiya D, Egawa N, Sakamoto T, Kikkawa Y, Shinkawa T, Isobe T, Koshikawa N, Seiki M
The Journal of biological chemistry 2009 Oct 2;284(40):27360-9
The Journal of biological chemistry 2009 Oct 2;284(40):27360-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human BCAM by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line, human prostate tissue, human kidney tissue, and human thyroid tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human BCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF148) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for BCAM at approximately 70-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
