Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- PAB6227 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6227, RRID:AB_1576405
- Product name
- IRF5 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of IRF5.
- Antigen sequence
C-QGPWPMHPAGMQ
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Interferon regulatory factor 5 represses expression of the Epstein-Barr virus oncoprotein LMP1: braking of the IRF7/LMP1 regulatory circuit.
Ning S, Huye LE, Pagano JS
Journal of virology 2005 Sep;79(18):11671-6
Journal of virology 2005 Sep;79(18):11671-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- IRF5 polyclonal antibody (Cat # PAB6227) staining (2 ug/mL) of A-549 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HEK293 overexpressing human IRF5 and probed with IRF5 polyclonal antibody (Cat # PAB6227) at 1 ug/mL (mock transfection in lane 2) .