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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [2]
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Validation data
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- Product number
- GTX632010 - Provider product page
- Provider
- GeneTex
- Product name
- SA1 antibody [GT1515]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (GTX632010) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- STAG1 antibody detects STAG1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with STAG1 antibody (GTX632010) diluted by 1:1000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody (GTX632010) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (GTX632010) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SA1 antibody [GT1515] detects SA1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SA1 protein stained by SA1 antibody [GT1515] (GTX632010) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of STAG1 protein from HeLa whole cell extracts using 5 ?g of STAG1 antibody [GT1515] (GTX632010).Western blot analysis was performed using STAG1 antibody [GT1515] (GTX632010).EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
- Validation comment
- IP
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of SA1 protein from HeLa whole cell extracts using 5 £gg of SA1 antibody [GT1515] (GTX632010).Western blot analysis was performed using SA1 antibody [GT1515] (GTX632010).EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
