Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB400-153 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB400-153, RRID:AB_10001255
- Product name
- Rabbit Polyclonal FXR/NR1H4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 2.4 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Activated-farnesoid X receptor (FXR) expressed in human sperm alters its fertilising ability.
Mice lacking ARV1 have reduced signs of metabolic syndrome and non-alcoholic fatty liver disease.
The farnesoid X receptor regulates transcription of 3beta-hydroxysteroid dehydrogenase type 2 in human adrenal cells.
Malivindi R, Santoro M, De Rose D, Panza S, Gervasi S, Rago V, Aquila S
Reproduction (Cambridge, England) 2018 Sep;156(3):249-259
Reproduction (Cambridge, England) 2018 Sep;156(3):249-259
Mice lacking ARV1 have reduced signs of metabolic syndrome and non-alcoholic fatty liver disease.
Gallo-Ebert C, Francisco J, Liu HY, Draper R, Modi K, Hayward MD, Jones BK, Buiakova O, McDonough V, Nickels JT Jr
The Journal of biological chemistry 2018 Apr 20;293(16):5956-5974
The Journal of biological chemistry 2018 Apr 20;293(16):5956-5974
The farnesoid X receptor regulates transcription of 3beta-hydroxysteroid dehydrogenase type 2 in human adrenal cells.
Xing Y, Saner-Amigh K, Nakamura Y, Hinshelwood MM, Carr BR, Mason JI, Rainey WE
Molecular and cellular endocrinology 2009 Feb 27;299(2):153-62
Molecular and cellular endocrinology 2009 Feb 27;299(2):153-62
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FXR Antibody [NB400-153] - Detection of FXR in HeLa nuclear extract using NB400-153. ECL 30 minute detection.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FXR/NR1H4 Antibody [NB400-153] - Total protein from human A549, U2OS cell and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-FXR (NB400-153) in block buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).
