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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX102175 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102175, RRID:AB_1240592
- Product name
- PAF49 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: H1299 7.5% SDS PAGE GTX102175 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PAF49 antibody detects PAF49 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with PAF49 antibody (GTX102175) diluted by 1:1000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with PAF49 antibody (GTX102175) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with PAF49 antibody (GTX102175) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PAF49 antibody detects PAF49 protein at nucleolus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PAF49 protein stained by PAF49 antibody (GTX102175) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PAF49 antibody detects PAF49 protein at nucleolus by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: PAF49 protein stained by PAF49 antibody (GTX102175) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PAF49 antibody detects PAF49 protein at cytoplasm and nucleus in human ovarian cancer by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. PAF49 antibody (GTX102175) diluted at 1:500.
