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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00009412-M05 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00009412-M05, RRID:AB_894299
- Product name
- SURB7 monoclonal antibody (M05), clone 4B5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant SURB7.
- Antigen sequence
MADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASF
NNIQTAINKDQPANPTEEYAQLFAALIARTAKDID
VLIDSLPSEESTAALQAASLYKLEEENHEAATCLE
DVVYRGDMLLEKIQSALADIAQSQLKTRSGTHSQS
LPDS- Isotype
- IgG
- Antibody clone number
- 4B5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of MED21 expression in transfected 293T cell line by SURB7 monoclonal antibody (M05), clone 4B5.Lane 1: MED21 transfected lysate (Predicted MW: 15.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SURB7 is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to SURB7 on HepG2 cell. [antibody concentration 35 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
