Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- TA800737 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA800737, RRID:AB_2625650
- Product name
- HIF3A mouse monoclonal antibody,clone OTI2F7
- Antibody type
- Monoclonal
- Description
- HIF3A mouse monoclonal antibody,clone OTI2F7
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HIF3A
- Isotype
- IgG
- Antibody clone number
- OTI2F7
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIF3A (RC210255, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIF3A.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HIF3A monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Dilution: 1:500
- Validation comment
- WB