H00000837-M02

antibody from Abnova Corporation

Targeting: CASP4 ICE(rel)II, ICH-2, TX

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000837-M02

Provider

Abnova Corporation

Product name

CASP4 monoclonal antibody (M02), clone 7G7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant CASP4.

Antigen sequence

MAEGNHRKKPLKVLESLGKDFLTGVLDNLVEQNVL
NWKEEEKKKYYDAKTEDKVRVMADSMQEKQRMAGQ
MLLQTFFNIDQISPNKKAHPNMEAGPPESGESTDA
LKLCPHEEFLRLCKERAEEIYPIKERNNRTRLALI
ICNTEFDHLPPRNGADFDITGMKELLEGLDYSVDV
EENLTARDMESALRAFATRPEHKSSDSTFLVLMSH
GILEGICGTVHDEKKPDVLLYDTIFQIFNNRNCLS
LKDKPKVIIVQACRGANRGELWVRDSPASLEVASS
QSSENLEEDAVYKTHVEKDFIAFCSSTPHNVSWRD
STMGSIFITQLITCFQKYSWCCHLEEVFRKVQQSF
ETPRAKAQMPTIERLSMTRYFYLFPGN


Isotype

IgG

Antibody clone number

7G7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CASP4 monoclonal antibody (M02), clone 7G7. Western Blot analysis of CASP4 expression in HeLa.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CASP4 expression in transfected 293T cell line by CASP4 monoclonal antibody (M02), clone 7G7.Lane 1: CASP4 transfected lysate (Predicted MW: 43.3 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CASP4 is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol