Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-77045 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLSCR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PLSCR1 in extracts of various cell lines. Samples were incubated with PLSCR1 polyclonal antibody (Product # PA5-77045).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PLSCR1 Polyclonal Antibody (Product # PA5-77045) and a 35 kDa band corresponding to phospholipid scramblase 1 was observed across positive cell lines (THP 1, HaCaT, HCT 116 and Hep G2); and not across negative cell lines (SW480 and SH-SY5Y) tested. Membrane enriched extracts (30 µg lysate) of THP-1 (Lane 1), SW480 (Lane 2), HaCaT (Lane 3), HCT 116 (Lane 4), Hep G2 (Lane 5) and SH-SY5Y (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PLSCR1 in HeLa cells. Samples were incubated with PLSCR1 polyclonal antibody (Product # PA5-77045) followed by DAPI (nuclear staining).