Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-21760 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TOM1L1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, Molt-4, Raji, TOM1L1-transfected 293T.
- Concentration
- 0.8 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TOM1L1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a TOM1L1 Polyclonal Antibody (Product # PA5-21760) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TOM1L1 Polyclonal Antibody (Product # PA5-21760). Sample (30 µg of whole cell lysate). Lane A: Raji. 10% SDS PAGE. TOM1L1 Polyclonal Antibody (Product # PA5-21760) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TOM1L1 in methanol-fixed HeLa cells using a TOM1L1 polyclonal antibody (Product # PA5-21760) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using TOM1L1 (Product # PA5-21760) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:2,000.
