orb20665

antibody from Biorbyt

Targeting: LPO SPO

No application information provided.

Antibody Data

Product number

orb20665

Provider

Biorbyt

Proper citation

Biorbyt Cat#orb20665, RRID:AB_10753706

Product name

Lactoperoxidase antibody (Biotin)

Antibody type

Polyclonal

Description

Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to bovine lactoperoxidase. In immunocytochemical and immunohistochemical staining to identify and measure lactoperoxidase at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. Working dilutions for histochemical use are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:5,000.

Reactivity

Bovine

Host

Rabbit

Conjugate

Biotin

Vial size

1 ml

Concentration

IgG protein concentration 1 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 8.9. No foreign proteins added.

Storage

The lyophilised immunoconjugate is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. Reconstitute the lyophilised antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Diluted antiserum should be stored at 4°C, not refrozen, and preferably used the same day.