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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00004837-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004837-M03, RRID:AB_2153577
- Product name
- Mouse Monoclonal Nicotinamide N-Methyltransferase/NNMT Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. NNMT (2F2)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (2F2) [H00004837-M03] - Analysis of NNMT expression in transfected 293T cell line by NNMT monoclonal antibody (M03), clone 2F2. Lane 1: NNMT transfected lysatE (29.6 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Nicotinamide N-Methyltransferase/NNMT Antibody (2F2) [H00004837-M03] - Detection limit for recombinant GST tagged NNMT is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Nicotinamide N-Methyltransferase/NNMT Antibody (2F2) [H00004837-M03] - Analysis of NNMT transfected lysate using anti-NNMT monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NNMT rabbit polyclonal antibody.
