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antibody from Invitrogen Antibodies
Targeting: KCNAB1
AKR6A3, hKvb3, hKvBeta3, KCNA1B, Kvb1.3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- PA5-109346 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KVbeta1 (KCNAB1) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Mouse brain tissue lysates, rat brain tissue lysates, rat cerebellum tissue lysates, EA.hy926, rat testis tissue, rat brain tissue, rat kidney tissue, rat cerebellum tissue, JAR.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KCNAB1 in Positive control: Lane 1: mouse brain tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat cerebellum tissue lysates. Samples were transferred to PVDF membrane, blocked with 5% BSA (1 hour), incubated with KCNAB1 polyclonal antibody (Product # PA5-109346), at a dilution of 1:500, followed by Goat Anti-Rabbit IgG-HRP (1 hour) with a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KCNAB1 in Positive control: Lane 1: mouse brain tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat cerebellum tissue lysates. Samples were transferred to PVDF membrane, blocked with 5% BSA (1 hour), incubated with KCNAB1 polyclonal antibody (Product # PA5-109346), at a dilution of 1:500, followed by Goat Anti-Rabbit IgG-HRP (1 hour) with a dilution of 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of KCNAB1 in EA.hy926 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with KCNAB1 polyclonal antibody (Product # PA5-109346) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KCNAB1 in paraffin-embedded rat testis tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with KCNAB1 polyclonal antibody (Product # PA5-109346) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KCNAB1 in paraffin-embedded rat kidney tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with KCNAB1 polyclonal antibody (Product # PA5-109346) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KCNAB1 in paraffin-embedded rat cerebellum tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with KCNAB1 polyclonal antibody (Product # PA5-109346) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KCNAB1 in paraffin-embedded rat brain tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with KCNAB1 polyclonal antibody (Product # PA5-109346) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of KCNAB1 in JAR cells, unlabelled sample (control; cells without incubation with primary antibody; black). Samples were incubated with KCNAB1 polyclonal antibody (Product # PA5-109346) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG with a dilution of 1:1000 (30 min).
