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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-22254 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-XPB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-22254 targets XPB in ICC, IF, and WB applications and shows reactivity with Human samples. The PA5-22254 immunogen is a recombinant fragment corresponding to a region within amino acids 521 and 742 of XPB (Uniprot ID#P19447). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.69 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of XPB using A) 30 µg Neuro2A whole cell lysate (B) 30 µg GL261 whole cell lysate (C) 30 µg C8D30 whole cell lysate (D) 30 µg NIH-3T3 whole cell lysate (E) 30 µg BCL-1 whole cell lysate (F) 30 µg Raw264.7 whole cell lysate and G) 30 µg C2C12 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a XPB polyclonal antibody (Product # PA5-22254) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of XPB using 30 µg of A) 293T (B) A431 and C) H1299 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a XPB polyclonal antibody (Product # PA5-22254) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of XPB in paraformaldehyde-fixed HeLa cells using a XPB polyclonal antibody (Product # PA5-22254) at a 1:200 dilution.
