orb152302

antibody from Biorbyt

Targeting: HSF1 HSTF1

Western blot (Recommended by provider)

Antibody Data

Product number

orb152302

Provider

Biorbyt

Product name

HSF1 antibody (ATTO-633)

Antibody type

Polyclonal

Description

Rabbit polyclonal to HSF1 (ATTO-633). HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function. The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization. There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form . Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Red dye

Vial size

100 μg

Concentration

1 mg/ml

Storage

Store at 2-8°C and -20°C for one year. (Shipped on ice packs)