ABIN362219

antibody from antibodies-online

Targeting: HSF1 HSTF1

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

ABIN362219

Provider

antibodies-online

Product name

anti-Heat Shock Factor Protein 1 (HSF1) (pSer307) antibody

Antibody type

Polyclonal

Antigen

Peptide sequence around phosphorylation site of pSer307 (P-Q-S (p) -P-R) derived from Human HSF1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Reactivity

Human

Host

Rabbit

Epitope

pSer307

Isotype

IgG

Vial size

50 μL

Concentration

1 mg/mL

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

References

Submitted references

Analysis of phosphorylation of human heat shock factor 1 in cells experiencing a stress.

Guettouche T, Boellmann F, Lane WS, Voellmy R
BMC biochemistry 2005 Mar 11;6:4

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Regulation of molecular chaperone gene transcription involves the serine phosphorylation, 14-3-3 epsilon binding, and cytoplasmic sequestration of heat shock factor 1.

Wang X, Grammatikakis N, Siganou A, Calderwood SK
Molecular and cellular biology 2003 Sep;23(17):6013-26

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Rapid and reversible relocalization of heat shock factor 1 within seconds to nuclear stress granules.

Jolly C, Usson Y, Morimoto RI
Proceedings of the National Academy of Sciences of the United States of America 1999 Jun 8;96(12):6769-74