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Western blot
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- Antibody Data
- Antigen structure
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- Validations
- Other assay [2]
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Validation data
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- Product number
- STJ72774 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-SERCA2/ATP2A2 antibody (Internal) (STJ72774)
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody anti-SERCA2/ATP2A2 (Internal) is suitable for use in ELISA and Western Blot research applications.
- Reactivity
- Human, Mouse, Rat, Canine, Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
DELNPSAQRDACLN- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20 on receipt and minimise freeze-thaw cycles.
- Handling
- NA
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Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ72774 (0. 1µg/ml) staining of Human (A) , Mouse (B) and Rat (C) Heart lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ72774 mmunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
