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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NB100-81661 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-81661, RRID:AB_1145386
- Product name
- Rabbit Polyclonal DNA Ligase III Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNA Ligase III Antibody [NB100-81661] - Detection of Human DNL3 on HeLa whole cell lysate using NB100-81661. DNL3 was also immunoprecipitated by rabbit anti-DNL3 antibody NB100-81660.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNA Ligase III Antibody [NB100-81661] - Detection of Human DNL3 by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNL3 antibody NB100-81661 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: DNA Ligase III Antibody [NB100-81661] - Detection of human DNA Ligase III by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNA Ligase III antibody NB100-81661 used for IP at 6 ug per reaction. DNA Ligase III was also immunoprecipitated by rabbit anti-DNA Ligase III antibody from Company B. For blotting immunoprecipitated DNA Ligase III, NB100-81661 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
