TA801491

antibody from OriGene

Targeting: POLI RAD30B, RAD3OB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA801491

Provider

OriGene

Proper citation

OriGene Cat#TA801491, RRID:AB_2625967

Product name

POLI mouse monoclonal antibody, clone OTI3B5 (formerly 3B5)

Antibody type

Monoclonal

Description

POLI mouse monoclonal antibody, clone OTI3B5 (formerly 3B5)

Host

Mouse

Conjugate

Unconjugated

Epitope

POLI

Isotype

IgG

Antibody clone number

OTI3B5

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLI (RC207550, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLI.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-POLI monoclonal antibody.(1:200)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-POLI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801491)

Validation comment

IHC