PA5-89163

antibody from Invitrogen Antibodies

Targeting: NUDT1 MTH1

Immunoprecipitation

Antibody data

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Validation data

Product number: PA5-89163 - Provider product page
Provider: Invitrogen Antibodies
Product name: MTH1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: MSGISPQQMG EPEGSWSGKN PGTMGASRLY TLVLVLQPQR VLLGMKKRGF GAGRWNGFGG KVQEGETIED GARRELQEES GLTVDALHKV GQIVFEFVGE PELMDVHVFC TDSIQGTPVE SDEMRPCWFQ LDQIPFKDMW PDDSYWFPLL LQKKKFHGYF KFQGQDTILD YTLREVDTV; Positive Samples: SW480, K562, 22RV1, THP-1, HepG2, SKOV3, 293T; Cellular Location: Cytoplasm, Mitochondrion matrix, Nucleus
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.94 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

NUDT1 protein structure - PA5-89163 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of extracts of various cell lines, using NUDT1 Polyclonal antibody (Product # PA5-89163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 10s.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of extracts from normal (control) and NUDT1 knockout (KO) 293T cells, using NUDT1 Polyclonal antibody (Product # PA5-89163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 10s.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of MTH1 in extracts from normal (control) and NUDT1 knockout (KO) 293T cells using MTH1 Polyclonal Antibody (Product # PA5-89163) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of MTH1 in extracts of various cell lines using MTH1 Polyclonal Antibody (Product # PA5-89163) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.