PA5-17299

antibody from Invitrogen Antibodies

Targeting: PRMT1 ANM1, HCP1, HRMT1L2

Antibody data

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Validation data

PRMT1 protein structure - PA5-17299 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PRMT1 in cell lysates from A549 and NIH/3T3 cells using PRMT1 polyclonal antibody (Product # PA5-17299).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of PRMT1 was achieved by transfecting HCT 116 cells with PRMT1 specific siRNAs (Silencer® select Product # s6918; s6917). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the PRMT1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with PRMT1 Polyclonal Antibody (Product # PA5-17299, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PRMT1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis was performed on modified whole cell extracts (15 SDS) (30 µg lysate) of HeLa (Lane 1), HCT116 (Lane 2), Caco-2 (Lane 3), A549 (Lane 4), and NIH/3T3 (Lane 5). The blot was probed with Anti-PRMT1 Polyclonal Antibody (Product # PA5-17299, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 42 kDa band corresponding to PRMT1 was detected in all human and mouse cell line(s) tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of PRMT1 in either untreated HeLa using a PRMT1 polyclonal antibody (Product # PA5-17299) (green). Actin filaments are labeled with a fluorescent red phalloidin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of PRMT1 in either untreated HCT-116 using a PRMT1 polyclonal antibody (Product # PA5-17299) (green). Actin filaments are labeled with a fluorescent red phalloidin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of PRMT1 in either untreated SK-OV-3 using a PRMT1 polyclonal antibody (Product # PA5-17299) (green). Actin filaments are labeled with a fluorescent red phalloidin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of PRMT1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PRMT1 Polyclonal Antibody (Product # PA5-17299) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing Nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.