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LearnMAB560
antibody from Novus Biologicals
Targeting: GFRA1
GDNFR, GDNFRA, GFR-ALPHA-1, RET1L, RETL1, TRNR1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB560 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal GFR alpha-1/GDNF R alpha-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects rat GFR alpha-1/GDNF R alpha-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Ret, recombinant human (rh) GFR alpha-2, rhGFR alpha-3 or rmGFR alpha-2 is observed.
- Reactivity
- Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM).
Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
Expression and function of GDNF family ligands and receptors in the carotid body.
Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin.
Euteneuer S, Yang KH, Chavez E, Leichtle A, Loers G, Olshansky A, Pak K, Schachner M, Ryan AF
Molecular and cellular neurosciences 2013 May;54:30-43
Molecular and cellular neurosciences 2013 May;54:30-43
Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
Kubota H, Wu X, Goodyear SM, Avarbock MR, Brinster RL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Aug;25(8):2604-14
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Aug;25(8):2604-14
Expression and function of GDNF family ligands and receptors in the carotid body.
Leitner ML, Wang LH, Osborne PA, Golden JP, Milbrandt J, Johnson EM Jr
Experimental neurology 2005 Feb;191 Suppl 1:S68-79
Experimental neurology 2005 Feb;191 Suppl 1:S68-79
Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin.
Carmillo P, Dagø L, Day ES, Worley DS, Rossomando A, Walus L, Orozco O, Buckley C, Miller S, Tse A, Cate RL, Rosenblad C, Sah DW, Grønborg M, Whitty A
Biochemistry 2005 Feb 22;44(7):2545-54
Biochemistry 2005 Feb 22;44(7):2545-54
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Rat GFR alpha-1/GDNF R alpha-1 by Western Blot. Western blot shows lysates of rat spinal cord tissue and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat GFR alpha-1/GDNF R alpha-1 Monoclonal Antibody (Catalog # MAB560) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GFR alpha-1/GDNF R alpha-1 at approximately 60 kDa (as indicated). This experiment was conducted under non-reducing conditions (recommended) and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- GFR alpha-1/GDNF R alpha-1 in Rat Spinal Cord. GFR alpha-1/GDNF R alpha-1 was detected in perfusion fixed frozen sections of rat spinal cord using Rat GFR alpha-1/GDNF R alpha-1 Monoclonal Antibody (Catalog # MAB560) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
