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Learn674603
antibody from BioLegend
Targeting: GZMB
CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 674603 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#674603, RRID:AB_2687057
- Product name
- Direct-Blot™ HRP anti-Granzyme B
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Antibody clone number
- M3304B06
- Vial size
- 100 µl
- Storage
- -20°C
- Handling
- Blue Ice
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Total cell lysate from HeLa (lane 1, negative control) and PBMC (lane 2) (15 µg/lane) were resolved by electrophoresis (4-12% Bis-Tris), transferred to nitrocellulose, and probed with 1:250 purified anti-Granzyme B (clone M3304B06) and 1:1000, 1:2500 and 1:10000 Direct-Blot™ HRP anti- Granzyme B Antibody(upper) or loading control GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for the purified antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M is the molecular weight ladder.
