14-8889-80

antibody from Invitrogen Antibodies

Targeting: GZMB CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

14-8889-80

Provider

Invitrogen Antibodies

Product name

Granzyme B Monoclonal Antibody (496B), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The 496B antibody reacts with human Granzyme B (GrB). GrB is one of the family of serine proteases, known as granzymes, that is located in the granules of cytotoxic T cells and NK cells. Granzyme B has been described also as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation. GrB has been called CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. GrB is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved has been identified as mannose 6-phosphate receptor. This receptor functions as a death receptor for granzyme B during cytotoxic T cell-induced apoptosis.

Antibody clone number

496B

Concentration

0.5 mg/mL

References

Submitted references

Improved Purification of Human Granzyme A/B and Granulysin Using a Mammalian Expression System.

Rasi V, Hameed OA, Matthey P, Bera S, Grandgenett DP, Salentinig S, Walch M, Hoft DF
Frontiers in immunology 2022;13:830290

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Canine Natural Killer Cell-Derived Exosomes Exhibit Antitumor Activity in a Mouse Model of Canine Mammary Tumor.

Lee J, Lee SA, Gu NY, Jeong SY, Byeon JS, Jeong DU, Ouh IO, Lee YH, Hyun BH
BioMed research international 2021;2021:6690704

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Remodeling of secretory lysosomes during education tunes functional potential in NK cells.

Goodridge JP, Jacobs B, Saetersmoen ML, Clement D, Hammer Q, Clancy T, Skarpen E, Brech A, Landskron J, Grimm C, Pfefferle A, Meza-Zepeda L, Lorenz S, Wiiger MT, Louch WE, Ask EH, Liu LL, Oei VYS, Kjällquist U, Linnarsson S, Patel S, Taskén K, Stenmark H, Malmberg KJ
Nature communications 2019 Jan 31;10(1):514

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Granzymes: exogenous proteinases that induce target cell apoptosis.

Smyth MJ, Trapani JA
Immunology today 1995 Apr;16(4):202-6

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Immunohistochemical localization of granzyme B antigen in cytotoxic cells in human tissues.

Hameed A, Truong LD, Price V, Kruhenbuhl O, Tschopp J
The American journal of pathology 1991 May;138(5):1069-75

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Human natural killer cells: biologic and pathologic aspects.

Trinchieri G, Perussia B
Laboratory investigation; a journal of technical methods and pathology 1984 May;50(5):489-513

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of NK-92 (Lane 1) and NK-92 treated with IL-2 (100 ng/mL for 48 hours) (Lane 2). The blot was probed with Anti-Granzyme B Monoclonal Antibody (496B) (Product # 14-8889-82, 1 µg/mL) and detected by chemiluminescence using F(ab')2-Rabbit anti-Rat IgG (H+L) Secondary Antibody, HRP conjugate (Product # PA1-29927, 0.25 µg/mL, 1:4000 dilution). A 32 kDa band corresponding to Granzyme B was observed in the cell line tested and was enhanced upon treatment.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue using 5 µg/mL Rat IgG2a K Isotype Control Purified (left) or 5 µg/mL Anti-Human Human Granzyme B Purified (right) followed by Anti-Rat IgG Biotin and DAB visulization.Nuclei are stained with hematoxylin.