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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00001615-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001615-M01, RRID:AB_538159
- Product name
- Mouse Monoclonal DARS Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. DARS - aspartyl-tRNA synthetase
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Proteomic identification of putative biomarkers of radiotherapy resistance: a possible role for the 26S proteasome?
Proteomic identification of putative biomarkers of radiotherapy resistance: a possible role for the 26S proteasome?
Smith L, Qutob O, Watson MB, Beavis AW, Potts D, Welham KJ, Garimella V, Lind MJ, Drew PJ, Cawkwell L
Neoplasia (New York, N.Y.) 2009 Nov;11(11):1194-207
Neoplasia (New York, N.Y.) 2009 Nov;11(11):1194-207
Proteomic identification of putative biomarkers of radiotherapy resistance: a possible role for the 26S proteasome?
Smith L, Qutob O, Watson MB, Beavis AW, Potts D, Welham KJ, Garimella V, Lind MJ, Drew PJ, Cawkwell L
Neoplasia (New York, N.Y.) 2009 Nov;11(11):1194-207
Neoplasia (New York, N.Y.) 2009 Nov;11(11):1194-207
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DARS Antibody (2F11) [H00001615-M01] - Analysis of DARS over-expressed 293 cell line, cotransfected with DARS Validated Chimera RNAi ( Cat # H00001615-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DARS monoclonal antibody (M01), clone 2F11 (Cat # H00001615-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DARS Antibody (2F11) [H00001615-M01] - Analysis of DARS expression in transfected 293T cell line by DARS monoclonal antibody (M01), clone 2F11.Lane 1: DARS transfected lysate(57.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DARS Antibody (2F11) [H00001615-M01] - DARS monoclonal antibody (M01), clone 2F11 Analysis of DARS expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: DARS Antibody (2F11) [H00001615-M01] - Detection limit for recombinant GST tagged DARS is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DARS Antibody (2F11) [H00001615-M01] - Analysis of monoclonal antibody to DARS on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
