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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
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Validation data
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- Product number
- MA1-23228 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FEN1 Monoclonal Antibody (4E7)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- Recommended positive controls: MCF7.
- Antibody clone number
- 4E7
- Concentration
- 1.73 mg/mL
Submitted references PARP inhibition impedes the maturation of nascent DNA strands during DNA replication.
Vaitsiankova A, Burdova K, Sobol M, Gautam A, Benada O, Hanzlikova H, Caldecott KW
Nature structural & molecular biology 2022 Apr;29(4):329-338
Nature structural & molecular biology 2022 Apr;29(4):329-338
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FEN1 using 30 µg of MCF-7 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a FEN1 monoclonal antibody (Product # MA1-23228) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FEN1 in whole cell extract (30 µg). Samples was separated by 12% SDS-PAGE and the membrane was probed with FEN1 Monoclonal antibody (Product # MA1-23228) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FEN1 Monoclonal Antibody (4E7) (Product # MA1-23228). Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with FEN1 Polyclonal Antibody FEN1 Monoclonal Antibody (4E7) (Product # MA1-23228) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of FEN1 in paraformaldehyde-fixed MCF7 cells using a FEN1 monoclonal antibody (Product # MA1-23228) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed MCF7, using FEN1 [4E7] (Product # MA1-23228) antibody at 1:200 dilution.
