NB100-320

antibody from Novus Biologicals

Targeting: FEN1 FEN-1, MF1, RAD2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-320

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-320, RRID:AB_10002232

Product name

Rabbit Polyclonal FEN-1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

YY1 suppresses FEN1 over-expression and drug resistance in breast cancer.

Wang J, Zhou L, Li Z, Zhang T, Liu W, Liu Z, Yuan YC, Su F, Xu L, Wang Y, Zhou X, Xu H, Hua Y, Wang YJ, Zheng L, Teng YE, Shen B
BMC cancer 2015 Feb 13;15:50

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Okazaki fragment processing-independent role for human Dna2 enzyme during DNA replication.

Duxin JP, Moore HR, Sidorova J, Karanja K, Honaker Y, Dao B, Piwnica-Worms H, Campbell JL, Monnat RJ Jr, Stewart SA
The Journal of biological chemistry 2012 Jun 22;287(26):21980-91

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FEN1 ensures telomere stability by facilitating replication fork re-initiation.

Saharia A, Teasley DC, Duxin JP, Dao B, Chiappinelli KB, Stewart SA
The Journal of biological chemistry 2010 Aug 27;285(35):27057-66

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Human flap endonuclease I is in complex with telomerase and is required for telomerase-mediated telomere maintenance.

Sampathi S, Bhusari A, Shen B, Chai W
The Journal of biological chemistry 2009 Feb 6;284(6):3682-90

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Long patch base excision repair in mammalian mitochondrial genomes.

Szczesny B, Tann AW, Longley MJ, Copeland WC, Mitra S
The Journal of biological chemistry 2008 Sep 26;283(39):26349-56

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Physical and functional interaction between human oxidized base-specific DNA glycosylase NEIL1 and flap endonuclease 1.

Hegde ML, Theriot CA, Das A, Hegde PM, Guo Z, Gary RK, Hazra TK, Shen B, Mitra S
The Journal of biological chemistry 2008 Oct 3;283(40):27028-37

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Telomere repeat binding factor 2 interacts with base excision repair proteins and stimulates DNA synthesis by DNA polymerase beta.

Muftuoglu M, Wong HK, Imam SZ, Wilson DM 3rd, Bohr VA, Opresko PL
Cancer research 2006 Jan 1;66(1):113-24

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Tyrosine phosphorylation controls PCNA function through protein stability.

Wang SC, Nakajima Y, Yu YL, Xia W, Chen CT, Yang CC, McIntush EW, Li LY, Hawke DH, Kobayashi R, Hung MC
Nature cell biology 2006 Dec;8(12):1359-68

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FEN-1 Antibody [NB100-320] - Detection of Human and Mouse Fen1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Fen1 antibody NB100-320 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FEN-1 Antibody [NB100-320] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T, 50 ug) cells. Affinity purified rabbit antiFen1 antibody used for WB at 0.1 ug/ml.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FEN-1 Antibody [NB100-320] - Analysis of human Fen1, using NB100-320. Sample: Whole cell lysate (20 ug) from HeLa cells separated on a 4-12% PAGE gel.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: FEN-1 Antibody [NB100-320] - Whole cell lysate from HeLa cells (1 mg loaded), affinity purified rabbit antiFen1 antibody used for IP at 3 ug/mg lysate. Fen1 was also immunoprecipitated by rabbit anti-Fen1 antibody NB100-321, which recognizes a downstream epitope.