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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-78302 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZEB2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: MEF, A375, Neuro 2A, C8D30, Raw264.7, C2C12, rat brain
- Concentration
- 0.42 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZEB2 in whole cell lysate using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with ZEB2 polyclonal antibody (Product # PA5-78302) using a dilution of 1:1000 followed by HRP-conjugated anti-rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZEB2 in whole cell lysate using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with ZEB2 polyclonal antibody (Product # PA5-78302) using a dilution of 1:500 followed by HRP-conjugated anti-rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ZEB2 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ZEB2 Polyclonal Antibody (Product # PA5-78302) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZEB2 was performed by separating 30 µg of mouse tissue extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ZEB2 Polyclonal Antibody (Product # PA5-78302) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ZEB2 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ZEB2 Polyclonal Antibody (Product # PA5-78302) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZEB2 was performed by separating 30 µg of whole cell extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ZEB2 Polyclonal Antibody (Product # PA5-78302) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ZEB2 in A375 cells. Samples were treated with 4% paraformaldehyde at RT for 15 min and incubated with ZEB2 polyclonal antibody (Product # PA5-78302) using a dilution of 1:500, followed by Hoechst.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ZEB2 Polyclonal Antibody detects ZEB2 protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZEB2 stained by ZEB2 Polyclonal Antibody (Product # PA5-78302) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (Product # MA5-31466) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ZEB2 Polyclonal Antibody detects ZEB2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. ZEB2 stained by ZEB2 Polyclonal Antibody (Product # PA5-78302) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
