Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-21975 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Kir3.1 (KCNJ3) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
- Concentration
- 0.7 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GIRK1 using 30 µg of A) A431 (B) H1299 and C) HeLa lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a GIRK1 polyclonal antibody (Product # PA5-21975) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Kir3.1 (KCNJ3) Polyclonal Antibody (Product # PA5-21975). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Kir3.1 (KCNJ3) Polyclonal Antibody (Product # PA5-21975) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded DU145 xenograft, using GIRK1 (Product # PA5-21975) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
