NBP1-47276

antibody from Novus Biologicals

Targeting: FAF1 CGI-03, hFAF1, HFAF1s, UBXD12, UBXN3A

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-47276

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-47276, RRID:AB_10010230

Product name

Rabbit Polyclonal FAF1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FAF1 Antibody [NBP1-47276] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FAF1 antibody NBP1-47276 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. FAF1 was also immunoprecipitated by rabbit anti-FAF1 antibody NBP1-47277, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: FAF1 Antibody [NBP1-47276] - Sample: FFPE section of human ovarian carcinoma (left) and human colon carcinoma (right). Antibody: Affinity purified rabbit anti- FAF1 used at a dilution of 1:200 (1ug/ml). Detection: DAB