Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Other assay [1]
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Validation data
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- Product number
- PA5-17104 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 14-3-3 epsilon Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 34 µg/mL
- Storage
- -20°C
Submitted references Ago HITS-CLIP expands understanding of Kaposi's sarcoma-associated herpesvirus miRNA function in primary effusion lymphomas.
Haecker I, Gay LA, Yang Y, Hu J, Morse AM, McIntyre LM, Renne R
PLoS pathogens 2012;8(8):e1002884
PLoS pathogens 2012;8(8):e1002884
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 14-3-3 epsilon in extracts from Hela and NIH/3T3 cells using 14-3-3 epsilon polyclonal antibody (Product # PA5-17104).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-14-3-3 epsilon Polyclonal Antibody (Product # PA5-17104) and a ~29 kDa band was observed across the panel tested. Whole cell extracts (30 µg lysate) of Jurkat (Lane 1), PC-12 (Lane 2), SW 480 (Lane 3), HepG2 (Lane 4), HeLa (Lane 5), T-47D (Lane 6) and tissue extracts (30 µg lysate) of Mouse Brain (Lane 7), Mouse Testis (Lane 8), Mouse Skeletal Muscle (Lane 9), Rat Brain (Lane 10) and Rat Skeletal Muscle (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of YWHAE was achieved by transfecting SH-SY5Y cells with YWHAE specific siRNAs (Silencer® select Product # s200463). Western blot analysis (Fig. a) was performed using whole cell extracts from the SH-SY5Y knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with 14-3-3 epsilon Polyclonal Antibody (Product # PA5-17104, 1:5000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to YWHAE.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Western blot analysis of KSHV miRNA targets. Protein levels of two KSHV miRNA targets, TP53INP1 and YWHAE, were analyzed by Western blot in absence (0 ng) or presence (800 ng) of miR-K12-11 expression vector. Shown are two independent transfections of miR-K12-11. YWHAE showed moderate downregulation at the protein level, while TP53INP1 levels were reduced by more than 50% in the presence of miR-K12-11. Actin expression served as loading control.