Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- PA5-112825 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COBRA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 2.25 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of COBRA1 in multiple sample types using a COBRA1 polyclonal antibody (Product # PA5-112825) at a concentration of 2 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:15000. Sample types that were tested include: Lane 1: NIH/3T3 whole cell lysate, Lane 2: HL60 whole cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of COBRA1 in multiple sample types using a COBRA1 polyclonal antibody (Product # PA5-112825) at a concentration of 2 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:15000. Sample types that were tested include: Lane 1: NIH/3T3 whole cell lysate, Lane 2: HL60 whole cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of paraffin-embeded COBRA1 in human endometrial cancer using a COBRA1 Polyclonal antibody (Product # PA5-112825) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of paraffin-embeded COBRA1 in human colon cancer using a COBRA1 Polyclonal antibody (Product # PA5-112825) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation analysis of COBRA1 in 1.1*10^6 Hela cells using a COBRA1 polyclonal antibody (Product #PA5-112825). Hela cells were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 µg anti-NELFB or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the TFF1 promoter.