NBP1-06520

antibody from Novus Biologicals

Targeting: DICER1 Dicer, HERNA, K12H4.8-LIKE, KIAA0928, MNG1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-06520

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-06520, RRID:AB_1582360

Product name

Rabbit Polyclonal Dicer Antibody

Antibody type

Polyclonal

References

Submitted references

RNAase III-Type Enzyme Dicer Regulates Mitochondrial Fatty Acid Oxidative Metabolism in Cardiac Mesenchymal Stem Cells.

Su X, Jin Y, Shen Y, Kim IM, Weintraub NL, Tang Y
International journal of molecular sciences 2019 Nov 7;20(22)

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Exosomal cargo including microRNA regulates sensory neuron to macrophage communication after nerve trauma.

Simeoli R, Montague K, Jones HR, Castaldi L, Chambers D, Kelleher JH, Vacca V, Pitcher T, Grist J, Al-Ahdal H, Wong LF, Perretti M, Lai J, Mouritzen P, Heppenstall P, Malcangio M
Nature communications 2017 Nov 24;8(1):1778

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The HIV-1 Nef protein binds argonaute-2 and functions as a viral suppressor of RNA interference.

Aqil M, Naqvi AR, Bano AS, Jameel S
PloS one 2013;8(9):e74472

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In vivo structure-function analysis of human Dicer reveals directional processing of precursor miRNAs.

Gurtan AM, Lu V, Bhutkar A, Sharp PA
RNA (New York, N.Y.) 2012 Jun;18(6):1116-22

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Clarifying mammalian RISC assembly in vitro.

Tan GS, Garchow BG, Liu X, Metzler D, Kiriakidou M
BMC molecular biology 2011 Apr 29;12:19

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Dicer Antibody [NBP1-06520] - Western Blot and Immunoprecipitation: Dicer antibody [NBP1-06520]- Whole cell lysate from 293T (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Affinity purified rabbit anti-Dicer antibody NBP1-06520 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. Dicer was also immunoprecipitated by rabbit anti-Dicer antibodies NBP1-06521 and another anti-Dicer antibodiey, which recognize downstream epitopes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Dicer Antibody [NBP1-06520] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiDicer antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: Dicer Antibody [NBP1-06520] - Detection of human Dicer by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Dicer antibody NBP1-06520 (lot NBP1-06520-5) used for IP at 6 ug per reaction. Dicer was also immunoprecipitated by a previous lot of this antibody (lot NBP1-06520-4). For blotting immunoprecipitated Dicer, NBP1-06520 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.