MAB1116

antibody from Novus Biologicals

Targeting: PPBP b-TG1, Beta-TG, CTAP3, CTAPIII, CXCL7, LA-PF4, LDGF, MDGF, NAP-2, NAP-2-L1, PBP, SCYB7, TGB, TGB1, THBGB1

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1116

Provider

Novus Biologicals

Product name

Mouse Monoclonal CXCL7/Thymus Chemokine-1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects rat CXCL7/Thymus Chemokine-1 in ELISAs and Western blots. In sandwich ELISAs, no cross-reactivity or interference with recombinant human CXCL5, recombinant mouse CXCL7/Thymus Chemokine-1, recombinant rat (rr) CXCL1/CINC1, rrCXCL2/CINC3, rrCXCL3/CINC-2 alpha, rrCXCL3/CINC-2 beta, or rrCXCL5 is observed.

Reactivity

Rat

Host

Mouse

Isotype

IgG

Vial size

500 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chemotaxis Induced by CXCL7/Thymus Chemokine-1 and Neutralization by Rat CXCL7/Thymus Chemokine-1 Antibody. Recombinant Rat CXCL7/Thymus Chemokine-1 (Catalog # 1116-CK) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL7/ Thymus Chemokine-1 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CXCL7/ Thymus Chemokine-1 Monoclonal Antibody (Catalog # MAB1116). The ND₅₀ is typically 4-20 µg/mL.