AP09421PU-S

antibody from OriGene

Targeting: PRDM1 BLIMP1, PRDI-BF1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09421PU-S

Provider

OriGene

Product name

Rabbit Polyclonal Anti-PRDM1 Antibody, Aff - Purified

Antibody type

Polyclonal

Description

Rabbit Polyclonal Anti-PRDM1 Antibody, Aff - Purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

25ul

Concentration

1.15 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blots using affinity purified anti-PRDM1/BLIMP1 antibody show detection of overexpressed PRDM1/BLIMP1 in whole transfected Raji cell lysate (lane 2) at ~88kDa. Lane 1 shows mock transfection in whole Raji cell lysate. Detection of endogenous PRDM1/BLIMP1 (lane 4) is illustrated in human plasma cell nuclear extract, but not in Raji whole cell nuclear extract (lane 3). The identity of the lower dark band at ~50-60kDa is unknown. Primary antibody was used at a 1:1000 dilution in 5% PBS-Tween.

Validation comment

WB