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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AP09414PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09414PU-S, RRID:AB_2035829
- Product name
- anti SERPINB2 / PAI2
- Antibody type
- Polyclonal
- Antigen
- Peptide corresponding to an internal area of human PAI-2 protein
- Reactivity
- Human, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µl
- Concentration
- 1.2 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot using affinity purified anti-PAI-2 antibody shows detection of endogenous PAI-2 in 50 µg of HeLa whole cell lysates. The band at ~42 kDa (arrowhead) corresponds to PAI-2. Faint non-specific bands are also noted at lower and higher molecular weights positions. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1:5,000 dilution of HRP- conjugated Gta-Rabbit IgG for 45 min at room temperature with ECL used for detection. Molecular weight estimation was made by comparison to prestained MW markers.
