Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [3]
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Validation data
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- Product number
- MA5-13562 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD26 Monoclonal Antibody (202-36)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-13562 targets CD26 in FACS and IF applications and shows reactivity with Human and Rat samples. This antibody is not suitable for rat PC12 cells in FACS analysis.
- Antibody clone number
- 202-36
- Concentration
- 0.2 mg/mL
Submitted references Co-purification of Mac-2 binding protein with galectin-3 and association with prostasomes in human semen.
Heparan sulfate plays a central role in a dynamic in vitro model of protein-losing enteropathy.
Block AS, Saraswati S, Lichti CF, Mahadevan M, Diekman AB
The Prostate 2011 May 15;71(7):711-21
The Prostate 2011 May 15;71(7):711-21
Heparan sulfate plays a central role in a dynamic in vitro model of protein-losing enteropathy.
Bode L, Murch S, Freeze HH
The Journal of biological chemistry 2006 Mar 24;281(12):7809-15
The Journal of biological chemistry 2006 Mar 24;281(12):7809-15
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD26 in PBMC cells compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA5-13562) at a dilution of 0.25 µg/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4°C in the dark. FACS analysis was performed using 400 µL of cell buffer.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD26 in PBMC cells compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA5-13562) at a dilution of 0.25 µg/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4°C in the dark. FACS analysis was performed using 400 µL of cell buffer.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD26 in PC12 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde, washed with PBS, and incubated with CD26 monoclonal antibody (Product # MA5-13562) at a dilution of 0.25 µg/test for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.