Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- LS-C180211 - Provider product page
- Provider
- LSBio
- Product name
- SPTAN1 / Alpha Fodrin Antibody LS-C180211
- Antibody type
- Polyclonal
- Description
- Antiserum
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Main image
- Experimental details
- Western Blot: Alpha Fodrin Antibody - Western blot of mouse sciatic nerve extract (lanes 1 and 2) and brain homogenate (3). A prominent band running at 240kDa represents the intact Alpha Fodrin heavy chain. More minor bands below this likely represent in vivo proteolytic fragments of Alpha Fodrin.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunofluorescence: Alpha Fodrin Antibody - Mixed neuron-glial cultures stained with rabbit antibody to Alpha Fodrin (green) and mouse polyclonal antibody to Nestin (red). The Alpha Fodrin antibody stains numerous axonal and dendritic profiles in these cultures, clearly revealing the submembranous cytoskeleton. Since Alpha Fodrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody.