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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- PA5-110739 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAI3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LTKQRNPMDY PVEDAFCKPQ LVKKSYGVEN RAYSQEEITQ GFEETGDTLY APYSTHFQLQ NQPPQKEFSI PR
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAI3 in control (vector only transfected HEK293T lysate) and GPRC5A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401304) using RAI3 Polyclonal Antibody (Product # PA5-110739).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAI3 in human cell lines A-431 and U-251MG using RAI3 Polyclonal Antibody (Product # PA5-110739) antibody. Corresponding RAI3 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RAI3 in human cell line U-2 OS using RAI3 Polyclonal Antibody (Product # PA5-110739) shows localization to nucleoli, plasma membrane and vesicles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RAI3 in human lung and skeletal muscle tissues using RAI3 Polyclonal Antibody (Product # PA5-110739). Corresponding RAI3 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RAI3 in human small intestine tissue using RAI3 Polyclonal Antibody (Product # PA5-110739). Staining shows moderate positivity in apical membrane in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RAI3 in human urinary bladder tissue using RAI3 Polyclonal Antibody (Product # PA5-110739). Staining shows moderate membranous positivity in urothelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RAI3 in human lung tissue using RAI3 Polyclonal Antibody (Product # PA5-110739). Staining shows moderate membranous positivity in pneumocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RAI3 in human skeletal muscle tissue using RAI3 Polyclonal Antibody (Product # PA5-110739). Staining shows no positivity in myocytes as expected.
