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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-21544 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-PPP3CB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-21544 targets PPP3CB in IHC (P) and WB applications and shows reactivity with Human and mouse samples. The PA5-21544 immunogen is a recombinant fragment corresponding to a region within amino acids 63 and 393 of PPP3CB (Uniprot ID#P16298).
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.49 mg/ml
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PPP3CB using 50µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PPP3CB polyclonal antibody (Product # PA5-21544) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PPP3CB using 30µg of A) A431 and B) MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PPP3CB polyclonal antibody (Product # PA5-21544) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PPP3CB using 30µg of NIH-3T3 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PPP3CB polyclonal antibody (Product # PA5-21544) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PPP3CB showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a PPP3CB polyclonal antibody (Product # PA5-21544) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PPP3CB in paraffin-embedded SW480 xenograft using a PPP3CB polyclonal antibody (Product # PA5-21544) at a dilution of 1:500.
