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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- GTX80683 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX80683, RRID:AB_625362
- Product name
- Adiponectin antibody [19F1]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rabbit
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot detection of adiponectin in differentiated 3T3-L1 adipocytes.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot image of adiponectin in differentiated 3T3-L1 adipocytes using GTX80683.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on cancer biopsies of deparaffinized human colon carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with or without Adiponectin antibody [19F1] overnight at 4¢XC in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
