Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- UM500072 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM500072, RRID:AB_2629085
- Product name
- Adiponectin mouse monoclonal antibody,clone UMAB104
- Antibody type
- Monoclonal
- Description
- Adiponectin mouse monoclonal antibody,clone UMAB104
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ADIPOQ
- Isotype
- IgG
- Antibody clone number
- UMAB104
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human kidney tissue using ADIPOQ clone UMAB104, mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer pH6.0 was done at 120C for 3min in pressure chamber/cooker prior to UM500072 application. UM500072 was diluted 1:100 and detection with shown HRP enzyme and DAB chromogen. Strong cytoplasmic and membranous staining is seen in the glomeruli and vessel endothelia cells. Staining was not detected in the tubules.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded carcinoma of human lung tissue using ADIPOQ clone UMAB104, mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer pH6.0 was done at 120C for 3min in pressure chamber/cooker prior to UM500072 application. UM500072 was diluted 1:100 and detection shown with HRP enzyme and DAB chromogen. Strong cytoplasmic and membranous staining is seen in vessel endothelial cells no staining was seen in tumor cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ADIPOQ mouse monoclonal antibody. (UM500072; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded carcinoma of human bladder tissue using ADIPOQ clone UMAB104, mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer pH6.0 was done at 120C for 3min in pressure chamber/cooker prior to UM500072 application. UM500072 was diluted 1:100 and detection shown with HRP enzyme and DAB chromogen. Strong cytoplasmic and membranous staining is seen in vessel endothelial cells no staining was seen in tumor cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human lung using ADIPOQ clone UMAB104, mouse monoclonal antibody at 1:100 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM500072 requires heat-induced epitope retrieval with Citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong membranous and cytoplasmic staining of the endothelial cells. Normal lung epithelia cells and tumor cells are negative for ADIPOQ.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human placenta using ADIPOQ clone UMAB104, mouse monoclonal antibody at 1:100 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM500072 requires heat-induced epitope retrieval with Citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong membranous and cytoplasmic staining of the endothelial cells.
- Validation comment
- IHC