LF-MA0184

antibody from Invitrogen Antibodies

Targeting: HES1 bHLHb39, FLJ20408, HES-1, HRY

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

LF-MA0184

Provider

Invitrogen Antibodies

Product name

Anti-HES1 Monoclonal Antibody (7H11)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

A suggested positive control for this product is BeWo cells.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

7H11

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using HES1 Monoclonal Antibody (7H11) (Product # LF-MA0184) and a 48kDa band corresponding to HES1 was observed in the tested cell lines except IMR-32 and HL-60 as reported. Additional bands at ~80-90 kDa were also observed. Whole cell extracts (30 µg lysate) of OVCAR-3 (Lane 1), T47D (Lane 2), IMR-32 (Lane 3), HL-60 (Lane 4) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..