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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-47213 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NKp46 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, less than 5% cross-reactivity with recombinant mouse NKp46 (MAR-1) is observed and less than 1% cross-reactivity with recombinant human (rh) NKp30, rhNKp44, and rhNKp80 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of NKp46 in immersion fixedNK-92 human natural killer lymphoma cell line. Samples were incubated in NKp46 polyclonal antibody (Product # PA5-47213) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of NKp46 in immersion fixedNK-92 human natural killer lymphoma cell line. Samples were incubated in NKp46 polyclonal antibody (Product # PA5-47213) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of NKp46/NCR1 was detected in immersion fixedNK-92 human natural killer lymphoma cell line using Goat Anti-human NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47213) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Goat IgG Secondary Antibody (re and counterstained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NKp46 in immersion fixed paraffin-embedded sections of human lymph node. Samples were incubated with NKp46 polyclonal antibody (Product # PA5-47213) using a dilution of 3 µg/mL for 1 hour at room temperature followed by Anti-Goat IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm.
