Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- FAB1959G-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse IL-12 R beta 2 Alexa Fluor® 488-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse IL-12 R beta 2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human IL-1 R beta 1 is observed.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Green dye
- Antigen sequence
Q99665
- Isotype
- IgG
- Antibody clone number
- 305719
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Enhanced Acid Sphingomyelinase Activity Drives Immune Evasion and Tumor Growth in Non-Small Cell Lung Carcinoma.
Kachler K, Bailer M, Heim L, Schumacher F, Reichel M, Holzinger CD, Trump S, Mittler S, Monti J, Trufa DI, Rieker RJ, Hartmann A, Sirbu H, Kleuser B, Kornhuber J, Finotto S
Cancer research 2017 Nov 1;77(21):5963-5976
Cancer research 2017 Nov 1;77(21):5963-5976
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of IL-12 R beta 2 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 5 ng/mL Recombinant Human IL-12 (Catalog # 219-IL) and 10 μg/mLGoat Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA) for 3 days to induce Th1 development were stained with Mouse Anti-Human/Mouse IL-12 R beta 2 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1959G) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # IC002G). View our protocol for Staining Membrane-associated Proteins.