53-6400-82

antibody from Invitrogen Antibodies

Targeting: MYH11 SMHC, SMMHC

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

53-6400-82

Provider

Invitrogen Antibodies

Product name

Smooth Muscle Myosin Monoclonal Antibody (SMMS-1), Alexa Fluor™ 488, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: This SMMS-1 monoclonal antibody reacts with both isoforms of the human smooth muscle myosin heavy chain (SM1 and SM2) structural proteins, which are components of the contractile apparatus in smooth muscle cells. Smooth muscle myosin heavy chains are expressed by myoepithelial cells and also serve as the terminal differentiation marker for smooth muscle cells. In normal breast tissue, myoepithelial cells compose the outer layer of the lumen lining. In benign and in situ breast lesions, the intact myoepithelial layer stains with SMMS-1 and the loss of this staining indicates invasive cancer. This SMMS-1 antibody has also been reported to cross-react with bovine, canine, feline, and rat smooth muscle myosin heavy chains. Applications Reported: This SMMS-1 antibody has been reported for use in microscopy, immunocytochemistry, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This SMMS-1 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 30 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human, Rat, Bovine, Canine, Feline

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Antibody clone number

SMMS-1

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

4° C, store in dark, DO NOT FREEZE!

References

Submitted references

Loss of the serine protease HTRA1 impairs smooth muscle cells maturation.

Klose R, Prinz A, Tetzlaff F, Weis EM, Moll I, Rodriguez-Vita J, Oka C, Korff T, Fischer A
Scientific reports 2019 Dec 3;9(1):18224

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Contractile gene expression in HTRA1 -deficient VSMC is affected by Notch and TGFss signaling. ( a ) Representative photomicrographs of F-actin and immunolabeled myosin in HUASMC and quantification of F-actin and myosin positive areas (n = 4). ( b ) Representative photomicrographs of F-actin in HUASMC after four hours of TGFss inhibition using 10 uM ALK5 inhibitor (ALK5i, CAS 446859-33-2, Merck) or NOTCH3 inhibition for 16 hours using 25 uM DAPT (Merck) (n = 3). ( c - e ) Primary aortic VSMC derived from HtrA1 -/- or wildtype littermate controls were cultured until confluence was reached. Quantitative real-time PCR analysis of alpha smooth muscle actin (alpha-SMA). ( d , e ) VSMC were treated with DAPT (25 µM) for 16 hours or Alk5i (10 µM) for two hours before RNA isolation. Treatment with the solvent DMSO was used as control (n = 3). Statistical significance was determined by an unpaired student's t-test ( a,c,d,e ). *p < 0.05; **p < 0.01. Bar graphs show mean values, error bars indicate SD. Scale bar equals 10 um.

Conjugate

Green dye