NBP1-22974

antibody from Novus Biologicals

Targeting: SPHK1 SPHK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NBP1-22974

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-22974, RRID:AB_1726523

Product name

Rabbit Polyclonal Sphingosine Kinase 1/SPHK1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Inhibiting sphingosine kinase 2 mitigates mutant Huntingtin-induced neurodegeneration in neuron models of Huntington disease.

Moruno-Manchon JF, Uzor NE, Blasco-Conesa MP, Mannuru S, Putluri N, Furr-Stimming EE, Tsvetkov AS
Human molecular genetics 2017 Apr 1;26(7):1305-1317

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP1-22974] - SPHK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti- SPHK1 antibody NBP1-22974 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. SPHK1 was also immunoprecipitated by rabbit anti-SPHK1 antibody NBP1-22973, which recognizes an upstream epitope