Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- AM1817b - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AM1817b, RRID:AB_2227476
- Product name
- ATG16L1 Antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Purified Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.35 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Autophagy in cancer associated fibroblasts promotes tumor cell survival: Role of hypoxia, HIF1 induction and NFκB activation in the tumor stromal microenvironment.
Martinez-Outschoorn UE, Trimmer C, Lin Z, Whitaker-Menezes D, Chiavarina B, Zhou J, Wang C, Pavlides S, Martinez-Cantarin MP, Capozza F, Witkiewicz AK, Flomenberg N, Howell A, Pestell RG, Caro J, Lisanti MP, Sotgia F
Cell cycle (Georgetown, Tex.) 2010 Sep 1;9(17):3515-33
Cell cycle (Georgetown, Tex.) 2010 Sep 1;9(17):3515-33
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of anti-ATG16L1 Monoclonal Antibody (Cat.#AM1817b) by ATG16L1 recombinant protein (Fragment). ATG16L1 (Fragment) protein (arrow) was detected using the purified Mab. (1:2000)
- Primary Ab dilution
- 1:60
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ATG16L1 Monoclonal Antibody (Cat.#AM1817b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:10~50