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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP1817c - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP1817c, RRID:AB_636725
- Product name
- ATG16L Antibody (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Biochemical isolation and characterization of the tubulovesicular LC3-positive autophagosomal compartment.
Gao W, Kang JH, Liao Y, Ding WX, Gambotto AA, Watkins SC, Liu YJ, Stolz DB, Yin XM
The Journal of biological chemistry 2010 Jan 8;285(2):1371-83
The Journal of biological chemistry 2010 Jan 8;285(2):1371-83
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Western blot analysis of hAPG16L-K366 (Cat.#AP1817c) in NIH-3T3, HepG2, Hela, Jurkat and NCI-H460 cell line lysates (35ug/lane). APG16L (arrow) was detected using the purified Pab."
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:50~100
